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human lung cancer cell line hcc44  (DSMZ)


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    DSMZ human lung cancer cell line hcc44
    Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and <t>HCC44)</t> and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.
    Human Lung Cancer Cell Line Hcc44, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+cancer+cell+line+hcc44/HCC-44/pm26912193-249-0-10
    Average 95 stars, based on 59 article reviews
    human lung cancer cell line hcc44 - by Bioz Stars, 2026-09
    95/100 stars

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    1) Product Images from "Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome."

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome.

    Journal: Scientific reports

    doi: 10.1038/srep19857

    Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and HCC44) and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.
    Figure Legend Snippet: Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and HCC44) and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.

    Techniques Used: Mutagenesis, Expressing, Transfection, Plasmid Preparation

    Related Articles

    Mutagenesis:

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome.
    Article Snippet: The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.. Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.. Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome
    Article Snippet: The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.. Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.. Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).

    Expressing:

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome.
    Article Snippet: The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.. Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.. Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome
    Article Snippet: The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.. Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.. Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).

    Transfection:

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome.
    Article Snippet: The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.. Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.. Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome
    Article Snippet: The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.. Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.. Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).

    Plasmid Preparation:

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome.
    Article Snippet: The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.. Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.. Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome
    Article Snippet: The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.The human lung cancer cell lines A549, BEAS-2B, NCI-H441 (H441), NCI-H1299 (H1299), NCI-H1792 (H1792), and NCI-H2009 (H2009) cells were acquired from the American Type Culture Collection (ATCC), and maintained per the recommendation of ATCC.. Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.. Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).Human umbilical vein endothelial cells (HUVEC) were obtained from either Fisher Scientific (#8774388) or Life Technologies (#C-003-5C) and grown in Media 200 supplemented with low serum growth supplements (Life technologies, #S-003-10).



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    human lung cancer cell line hcc44  (DSMZ)
    95
    DSMZ human lung cancer cell line hcc44
    Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and <t>HCC44)</t> and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.
    Human Lung Cancer Cell Line Hcc44, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+cancer+cell+line+hcc44/HCC-44/pm26912193-249-0-10
    Average 95 stars, based on 1 article reviews
    human lung cancer cell line hcc44 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

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    Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and HCC44) and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.

    Journal: Scientific reports

    Article Title: Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome.

    doi: 10.1038/srep19857

    Figure Lengend Snippet: Figure 2. TTF-1 upregulates VEGF. Three dox-on TTF-1-inducible cells systems were hosted in two human lung AD cell lines (NCI-H1792 and HCC44) and a premalignant human lung epithelial cell line (BEAS-2B). A dox-on transgene of the HDD mutant of TTF-1 was also created in HCC44 and BEAS-2B cells. (A) TTF- 1− human lung AD cell line (A549) was used to host stable expression of either wt-TTF-1 or HDD mutant transgene. Protein expression of the dox-on TTF-1 transgene is shown in A, whereas the VEGF RNA (B,D,F,H) of the transfectant cells and the secreted VEGF protein in the corresponding CM (C,E,G,I) demonstrate VEGF upregulation by TTF-1. EV, empty vector.

    Article Snippet: Human lung cancer cell line HCC44 was obtained from the Leibniz Institute (DSMZ) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), penicillin and streptomycin.

    Techniques: Mutagenesis, Expressing, Transfection, Plasmid Preparation